Tag: Molecular biology
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PCR
Polymerase Chain Reaction Materials: DNA Template (sample to copy ) Test tube (cell nucleus not required) Buffers/Cofactors Primers Heat Thermostable DNA Polymerase like Taq Magnesium dNTPs Methods Many uses in Molecular biology all follow same principle steps: denaturation, annealing, and extension
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FastA
Algorithm developed 1985 by Lipman and Pearson Purpose: identify k-tuples in reference and comparison sequence Heuristic approach Mismatches in comparison k-tuples analogous to segment pairs in BLAST (Basic Local Alignment Search Tool)
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Phosphorylation state ratio
Electron flow Rp = ATP / (ADP*Pi) Kinases Phosphatases Proteins Post-translation non-synonymous SNPs (nsSNPs) PMC1208866
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Perfect Enzyme
◽︎ high Kcat ◽︎ low Km ◽︎ distinctive Kcat/Km ratio ◽︎ limited diffusion of substrate to product trap
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